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Module 3:  Enzymes

Laboratory 10:  Quantifying an enzyme reaction

                                

This laboratory concludes our module on the study of enzymes.  During the first laboratory of this module you learned how to obtain qualitative information on enzyme reactions.  During the second laboratory of this module you learned how to obtain quantitative information on the pH of various solutions.  Now you will learn how to obtain quantitative information on enzyme reactions, and you will examine a new enzyme/substrate combination.  Time will be spent learning about catechol and catechol oxidase (the substrate and enzyme with which you'll be working) and learning how to use the spectrophotometer to analyze this reaction. 

Textbook pages 148-149

Key Words: 

(You should be able to define or explain these terms.)
Spectrophotometer Wavelength Transmittance
Absorbance Dinitrosalicyclic Acid Maltose
Benzoquinone Catechol Catechol Oxidase
Cuvette Calibration of Spectrophotometer Blank
Control

Want to find out more about Enzyme Reactions?
Visit these sites:

 http://mvhs1.mbhs.edu/mvhsproj/enzyme/enzyme.html

http://www.cat.cc.md.us/biotutorials/proteins/enzsub.html

http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/enzymes.html

http://www.chm.davidson.edu/java/spec/spec.html

http://www.science-projects.com/Tyrosinase.htm

http://www.science-projects.com/CC101L5.htm

 

 

 

 

 

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