Richard Shand

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Designed by: Jeff Henrikson

BIO 349L / BIO 680

Molecular Techniques

Fall 2003

This course was made possible through funding from the National Sciences Foundation (NSF/CCLI grant #DUE-9981129; DUE-0311692), the Institute for Systems Biology, Seattle, WA, the College of Arts and Sciences and the Department of Biological Sciences of Northern Arizona University.

• General Course Description

•  Image Archive   

•  Syllabus

  • Syllabus and Course Schedule

  • Classroom Management Statement

  • NAU Policy Statements

  • Keeping a Laboratory Notebook

•   Protocols

  • Protocols for Week 1

  • Protocols for Week 2

  • Protocols for Week 3

  • Protocols for Week 4

  • Protocols for Week 5

  • Protocols for Week 6

  • Protocols for Week 7

  • Protocols for Week 8

  • Protocols for Week 9

  • Protocols for Week 10

  • Protocols for Week 11

  • Protocols for Week 14

  • T4 DNA Ligase

  • Halocin Activity Assay

  • Instructions for using the Shimadzu Sepctrophotometer

  • Preparing Top Agar Overlays of Halobacterium salinarum NRC817

  • Bio-Gel^® P (polyacrylamide gel)

  • Agarose primer

    • Agarose physical chemistry

    • Electrophoresis of DNA in agarose gels

    • Dissolving and casting agarose gels

    • Visualizing DNA in agarose gels

  • Silver Staining Tricine Gels (124 Kb)

  • Transformation with E. coli XL1-Blue Cells

•   Background Readings

  • Vreeland, R.H., W.D. Rosenzweig and D.W. Powers. 2000. Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal. Nature 407: 897 - 900.  [Full Text (.pdf)]

  • Price, L.B. and R. F. Shand. 2000. Halocin S8: A 36 amino acid microhalocin. J. Bact. 182: 4951-4958. [Full Text (html) (.pdf)]  

  • Schägger, Herman and Gebhard von Jagow. 1987. Tricine-Codium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis for the Separation  of Proteins in the Range from 1 to 100 kDa.  Analytical Biochemistry 166 368-379 [Full Text (.pdf) - 728Kb]  

  • Thompson, J.R., 1983. Camp Verde evaporites. The Mineralogical Record, 14 (#2): 85-90. [Full Text (.pdf) - 1.3 Mb]

  • Bloomfield, S.F., G.S.A.B. Stewart, C.E.R. Dodd, I.R. Booth and E.G.M. Power. 1998. The viable but non-culturable phenomenon explained? Microbiology, 144: 1-3. [Full Text (.pdf) - 180 Kb]

  • Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254. [Full Text (.pdf) - 229 Kb]

Last updated: 02/16/2006