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The EnGGen Facility provides automated sequencing and fragment
analysis using capillary-based AB 3130 and AB 3730xl DNA Analyzers
(instrumentation page). These instruments offer high throughput and
sensitivity with the ability to handle 400 to 800 samples per day.
Samples of single stranded, double stranded DNA, and PCR products,
can be sequenced with reads (on average) of 800 base pairs. Fragment
analysis can be performed for many marker methods including commonly
used methods such as AFLPs, MSATs, tRFLPs and SNPs.
Scheduling your 3130 or 3730xl run:
Instrument use is on a first-come,
first-served basis and users may sign up for a sequencing or fragment
analysis run using the Oracle
database calendar. Instructions on how to sign up for a 3130
or 3730xl run can be found here. |
Analysis Software:
For DNA sequencing we have AB Sequencing Analysis Software ver. 5.3.1,
which allows base calling, editing, trimming and printing for data
collection on both 3130 and 3730xl instruments. For fragment analysis
GeneMapper ver. 4.0 is available, which allows DNA sizing and allele
calling for data collected on either instruments. We currently have
6 licenses for GeneMapper. A new Biology Computing Lab scheduled
to come on-line in Fall 2008 will house 15 workstations, all of which
will run GeneMapper.
The following handouts and guides to Sequencing and Fragment Analysis
are available by clicking on the links below:
Automated Sequencing Chemistry Guide
Big Dye Cycle Sequencing ver. 3.1
GeneMapper AFLP Guide
GeneMapper Microsatellite Guide
GeneMapper SNPlex Guide
GeneMapper Quick Reference Guide
GeneMapper Reference Guide
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